THYMINELESS MUTAGENESIS IN BACTERIOPHAGE T4
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چکیده
منابع مشابه
Thymineless Mutagenesis in Bacteriophage
Thymine deprivation can be achieved in bacteriophage T4 either by the use of the thymidylate synthetase inhibitor FUdR, or by an appropriate combination of genetic blocks; both methods produce marked mutagenesis. Extensive tests of the specificity of thymineless mutagenesis reveal that only A:T base pairs are affected, and that transitions and possibly transversions are produced. This system th...
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Extracellular nonreplicating bacteriophage T4 particles accumulate mutations as functions of temperature, time, pH, and ionic environment via two mechanisms: 5-hydroxymethylcytidine deamination produces G.C----A.T transitions while a guanosine modification produces transversions. Neither frameshift mutations nor mutations at A.T base pairs are appreciably induced. We now show that heat induces ...
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ژورنال
عنوان ژورنال: Genetics
سال: 1973
ISSN: 1943-2631
DOI: 10.1093/genetics/74.3.393